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Adapted MS/MSALL Shotgun Lipidomics Approach for Analysis of Cardiolipin Molecular Species

WILEY AOCS (2017) 

Authors: Fei Gao · Justice McDaniel · Emily Y. Chen · Hannah E. Rockwell · Cindy Nguyen ·
Matthew D. Lynes · Yu-Hua Tseng · Rangaprasad Sarangarajan · Niven R. Narain ·
Michael A. Kiebish

Cardiolipin (Ptd2Gro) is a complex, doubly charged phospholipid located in the inner mitochondrial membrane where it plays an essential role in regulating bio-energetics. Abnormalities in Ptd2Gro content or composi-tion have been associated with mitochondrial dysfunction in a variety of disease states. Here, we report the develop-ment of an adapted high-resolution data-independent acqui-sition (DIA) MS/MSALL shotgun lipidomic method to enhance the accuracy and reproducibility of Ptd2Gro molecular species quantitation from biological samples. Utilizing the doubly charged molecular ions and the isoto-pic pattern with negative mode electrospray ionization mass spectrometry (ESI-MS) using an adapted MS/MSALL approach, we profiled more than 150 individual Ptd2Gro species, including monolysocardiolipin (MLPtd2Gro). The method described in this study demonstrated high reproduc-ibility, sensitivity, and throughput with a wide dynamic range. This high-resolution MS/MSALL shotgun lipidomics approach could be extended to screening aberrations of Ptd2Gro metabolism involved in mitochondrial dysfunction in various pathological conditions and diseases.

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